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CDMO Services

Bioanalytics

Bioanalytics and Protein Characterization Expertise

Supporting today’s ideas and tomorrow’s biotherapeutic solutions

As developers explore an increasingly diverse array of biotherapeutic molecule types and configurations, a partner with the capacity for efficient turnaround, state-of-the-art instrumentation and techniques, and a highly-skilled bioanalytics and protein characterization team is needed. Our bioanalytics team is here to meet these demands.

While clients trust us to deliver dependable and reliable bioanalytical services, we’ve never accepted the status quo. Whether exploring alternative approaches to protein production via our FastPharming® plant-based protein expression system, using our Glycaneering Service℠ to tailor and control N-glycosylation for next-generation antibody engineering, or leveraging mass spec multi-attribute methodology to improve efficiency, we have a unique perspective on the world of biotherapeutics.

To support great biotherapeutic ideas, no matter the routes utilized and who is developing them, our bioanalytical services team is here to help advance biotherapeutic research and development.

Computer generated image of a molecule and bioanalysis equipment for analysis and characterization services by iBio.
Looking for a Bioanalytical Services or Protein Characterization Partner?

Our experienced bioanalytical team can analyze an array of sample types including:

Recombinant proteins

Monoclonal, polyclonal & bispecific antibodies

Bioconjugates

Oligonucleotides

Pegylated peptides

Antigens for subunit vaccine design

Virus-like particles (VLP)

Enzymes

Blood factors and cytokines

Bioanalytical Capabilities

Bioanalytical Capabilities
Equipment/Method
Data Samples
Bioanalytical Capabilities
Bioanalytical Capabilities
Intact Protein Analysis

Protein proteoform determination

  • Intact analysis of glycoforms
  • Intact analysis of protein alone (de-glycosylated)
Equipment/Method
Equipment/Method

Reverse phase HR/LCMS on a Sciex X500B TOF/5600 TOF mass spectrometer

Data Samples
Data Samples
Line graph with Intensity on y-axis from 1-100 in increments of 20 and Mass (kDa) on x-axis from 144-148. Lines peak at high intensities signifying detection of a protein. Different peaks indicate different glycoforms attached to the protein.
Line graph with Intensity (cps) on y-axis from 0.0e0-3.5e5 in increments of 1.0e4 cps and Time (min) on the x-axis from 0-33 in increments of 1 min. The sample is deglycosylated, so the high intensity peak indicates the protein homogeneity without glycoforms attached.
Bioanalytical Capabilities
Bioanalytical Capabilities

Middle-down characterization for monoclonal antibodies

  • Heavy chain and light chain analysis (HRMS and CZE)
  • iDes digest for analysis of Fab and Fc regions
Equipment/Method
Equipment/Method

Reverse phase HR/LCMS on a Sciex X500B TOF/5600 TOF mass spectrometer

Data Samples
Data Samples
Line graph with Intensity (cps) on the y-axis from 0-8000 in increments of 500 cps and Time (min) on the x-axis from 0-33 in increments of 1 min. Sample is digested into light chain (LC) and heavy chain (HC) fragments. The high intensity peaks indicate LC and HC forms.
Line graph. Absorbance at 220 nm (AU) on y-axis from 0.000-0.015, increments of 0.001. Time (min) on x-axis. High absorbency peaks indicate LC and HC and can distinguish HC non-glycosylated from HC glycosylated. Then compares these to the internal standard.
Line graph. Intensity (cps) on y-axis from 0.0e0-7.5e5 in increments of 5e4. Time (min) on x-axis from 0-33 in increments of 1 min. Sample is digested into Fc and Fab antibody fragments, therefore high intensity peaks indicate Fc and Fab fragments.
Bioanalytical Capabilities
Bioanalytical Capabilities
Bottom-up Proteomic Assays

Amino acid sequence confirmation

  • Identification of post-translational modifications
  • Peptide mass fingerprinting
  • Glycopeptide identification
Equipment/Method
Equipment/Method

Reverse phase HR/LCMS on a Sciex X500B TOF/5600 TOF mass spectrometer

Data Samples
Data Samples
Line graph. Intensity (cps) on y-axis from 0.0e0-3.8e7 in increments of 2.0e6. Time (min) on x-axis from 0-24 in increments of 1. The protein is cleaved into peptide pieces and the high intensity peaks indicate these peptide pieces to measure mass and confirm amino acid sequence.
Bioanalytical Capabilities
Bioanalytical Capabilities
N-glycan profiling

Released glycan analysis by HR/LCMS and CZE with LIF detection

Equipment/Method
Equipment/Method

HILIC HR/LCMS on a Sciex X500B TOF/5600 TOF mass spectrometer and LIF detection on Sciex CESI CZE unit.

Data Samples
Data Samples
Line graph with Intensity (cps) on the y-axis from 0-10000 in increments of 5000 cps and time (min) on the x-axis from 0-21 in increments of 1 min. The protein is deglycosylated and high intensity peaks indicate the glycoform(s) that was/were released from the protein.
Line graph with RFU on the y-axis from 0-40 in increments of 2.5 and Time (min) on the x-axis from 0-6 in increments of 0.2 min. The protein is deglycosylated and high intensity peaks indicate the glycoforms(s) that was/were released from the protein.

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